Serum, plasma, or synovial fluid samples are tested quantitatively for IgM, IgG and IgA to B. burgdorferi using kinetic antibody capture enzyme immunoassays (EIA). In addition, two immunoblots for IgG antibody will be performed using two strains of B. burgdorferi which differ in their outer surface protein expression, to eliminate problems due to vaccination with recombinant OspA Lyme vaccine. Results of immunoblotting will include images of the reaction strips (when interpreted as positive) and a listing of the polypeptides reactive with patient antibody. If the acute specimen is negative (i.e. antibodies undetectable) and the clinical history strongly suggests infection, collect a convalescent specimen 3-4 weeks later.
Specimen Requirements for Lyme Antibody Tests:
Type: Serum, EDTA Plasma, or Synovial Fluid
Volume: 2.0 ml
Minimum: 0.5 ml
Storage: Refrigerate at 2-8° C
Stability: 2 Weeks Refrigerated or Frozen at -20° C
Turnaround Time: 24 to 48 Hours From Receipt
Follow-up Lyme Antibody Analysis
Retest of original sample in parallel with a new sample (i.e. acute and convalescent) will be performed as described above.
Turnaround Time: Routinely 24 to 48 Hours From Receipt